Human/Mouse ADAMTS1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF5867

R&D Systems, part of Bio-Techne
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BAF5867
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AF5867
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG

View all ADAMTS1 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse ADAMTS1
Phe254-Cys725
Accession # P97857

Specificity

Detects human and mouse ADAMTS1 in Western blots. In Western blots, less than 5% cross‑reactivity with recombinant human (rh) ADAMTS4, rhADAMTSL1.2, and rhADAMTS15 is observed and less than 1% cross‑reactivity with rhADAMTSL2, rhADAMTS5, rhADAMTS8, rhADAMTS10, rhADAMTS12, rhADAMTS13, and rhADAMTS16 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human/Mouse ADAMTS1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human ADAMTS1 (Catalog # 2197-AD) and Recombinant Mouse ADAMTS1 (catalog # Catalog # 5867-AD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS1

ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1), also known as METH1, is the founding member of the family of secreted zinc proteases with a multidomain structure (1-3). The protein precursor consists of a signal peptide and the following domains: pro, catalytic, disintegrinlike, TS type 1 motif, cysteine rich, spacer and a variable number of thrombospondin type 1 motifs. Based on their substrate specificity, ADAMTS1 and associated family members may be key enzymes in the degradation of cartilage leading to inflammation and arthritis (4). It is an active protease cleaving alpha2macroglobulin (5), aggrecan (6), and versican (7). Compared to ADAMTS4 (aggrecanase 1) and ADAMTS5 (aggrecanase 2), the aggrecanase activity of ADAMTS1 is lower. However, its activity can be enhanced by the binding of a cofactor such as fibulin1 (8). ADAMTS1 is essential for normal growth and the structure and function of the kidneys, adrenal glands and female reproductive organs (9). It also plays an important role in atherosclerosis (10). It has been shown to inhibit endothelial cell proliferation by direct binding and sequestration of VEGF165 and to inhibit fibroblast migration at high concentrations by binding to FGF 2 (11, 12). The purified rmADAMTS1 starts at the N terminus of the catalytic domain and ends in the Cys-rich domain.

References

  1. Vazquez, F. et al. (1999) J. Biol. Chem. 274:23349.
  2. Kuno, K. et al. (1997) J. Biol. Chem. 272:556.
  3. Porter, S. et al. (2005) Biochem. J. 386:15.
  4. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
  5. Kuno, K. et al. (1999) J. Biol. Chem. 274:18821.
  6. Kuno, K. et al. (2000) FEBS Lett. 478:241.
  7. Russel, D. L. et al. (2003) J. Biol. Chem. 278:42330.
  8. Lee, N. et al. (2005) J. Biol. Chem. 280:34796.
  9. Shindo, T. et al. (2000) J. Clin. Invest. 105:1345.
  10. Wight, T.N. (2005) Arterioscler Thromb. Vasc. Biol. 25:12.
  11. Luque, A. et al. (2003) J. Biol. Chem. 278:23656.
  12. Krampert, M. et al. (2005) J. Biol. Chem. 280:23844.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 1

Alternate Names

C3-C5, METH1

Entrez Gene IDs

9510 (Human)

Gene Symbol

ADAMTS1

UniProt

P97857

Additional ADAMTS1 Products

Product Documents for Human/Mouse ADAMTS1 Biotinylated Antibody

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Certificate of Analysis

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Product Specific Notices for Human/Mouse ADAMTS1 Biotinylated Antibody

For research use only

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