Human/Mouse Alkaline Phosphatase/ALPL Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2909
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Alkaline Phosphatase/ALPL
Leu18-Phe524
Leu18-Phe524
Specificity
Detects human and mouse Alkaline Phosphatase/ALPL in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Alkaline Phosphatase/ALPL Antibody
Detection of Human and Mouse Alkaline Phosphatase/ALPL by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BG01V human embryonic stem cells, NTera-2 human testicular embryonic carcinoma cell line, and mouse kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse Alkaline Phosphatase/ALPL Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, BG01V human embryonic stem cells, NTera‑2 human testicular embryonic carcinoma cell line, and mouse kidney tissue
Sample: HeLa human cervical epithelial carcinoma cell line, BG01V human embryonic stem cells, NTera‑2 human testicular embryonic carcinoma cell line, and mouse kidney tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF2909 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Alkaline Phosphatase/ALPL
Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503‑524) is not present in the mature form.
References
- Le Du, M-H. and J.L. Millan (2002) J. Biol. Chem. 277:49808.
- Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1.
- Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.
Long Name
Alkaline Phosphatase Liver
Alternate Names
Akp2, AP-TNAP, HOPS, TNAP, TNSALP
Gene Symbol
ALPL
Additional Alkaline Phosphatase/ALPL Products
Product Documents for Human/Mouse Alkaline Phosphatase/ALPL Antibody
Product Specific Notices for Human/Mouse Alkaline Phosphatase/ALPL Antibody
For research use only
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