Human/Mouse AMPK beta2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3808
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 376938
Product Specifications
Immunogen
E. coli-derived recombinant human AMPK beta2
Met1-Ile272
Accession # O43741
Met1-Ile272
Accession # O43741
Specificity
Detects human and mouse AMPK beta2 in Western blots. No cross-reactivity with recombinant human AMPK beta1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human/Mouse AMPK beta2 Antibody
Detection of Human/Mouse AMPK beta2 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line and MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 2 µg/mL Rat Anti-Human/Mouse AMPK beta2 Monoclonal Antibody (Catalog # MAB3808) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human AMPK beta2 (0.5 ng) was included. A specific band for AMPK beta 2 was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse AMPK beta2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: A549 human lung carcinoma cell line and MRC-5 human embryonic lung fibroblast cell line
Sample: A549 human lung carcinoma cell line and MRC-5 human embryonic lung fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AMPK beta 2
AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha1, alpha2, beta1, beta2, gamma1, gamma2, gamma3), with all 12 combinations able to form complexes. The beta1 subunit is expressed at higher levels than the beta2subunit in liver, while beta2 is more abundant in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.
Long Name
AMP-activated Protein Kinase beta 2
Alternate Names
PRKAB2
Gene Symbol
PRKAB2
UniProt
Additional AMPK beta 2 Products
Product Documents for Human/Mouse AMPK beta2 Antibody
Product Specific Notices for Human/Mouse AMPK beta2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...