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Human/Mouse BCAP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4857

R&D Systems, part of Bio-Techne
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MAB4857
MAB4857-SP

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 501813

Product Specifications

Immunogen

E. coli-derived recombinant human BCAP
Met462-Leu650
Accession # Q6ZUJ8

Specificity

Detects endogenous human and mouse BCAP in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse BCAP Antibody

Detection of Human/Mouse BCAP antibody by Western Blot.

Detection of Human BCAP by Western Blot.

Western Blot shows lysates of RL human non-Hodgkin's lymphoma B cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human/Mouse BCAP Monoclonal Antibody (Catalog # MAB4857) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for BCAP at approximately 80 kDa and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human/Mouse BCAP Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: RL human non-Hodgkin's lymphoma B cell line and Daudi human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCAP

BCAP (B cell adaptor for phosphoinositide 3-kinase (PI3K)), also known as PI3KAP1, participates in linking the B cell antigen receptor (BCR) with the PI3K pathway. Tyrosine phosphorylation of BCAP by BCR-associated protein tyrosine kinases, such as Syk and Btk, generates binding sites for the p85 subunit of PI3K, resulting in activation of the PI3K pathway. Human and mouse BCAP contain 3 YXXM SH2 binding motifs for interaction with PI3K. BCAP null mice have deficient immune function, with B cells that exhibit enhanced apoptosis.

Long Name

B Cell Adaptor Protein

Alternate Names

Pik3ap1

Entrez Gene IDs

118788 (Human); 83490 (Mouse); 294048 (Rat)

Gene Symbol

PIK3AP1

UniProt

Additional BCAP Products

Product Documents for Human/Mouse BCAP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse BCAP Antibody

For research use only

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