Human/Mouse Bcl-w Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8241

R&D Systems, part of Bio-Techne
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MAB8241
MAB8241-SP
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 90336

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Product Summary for Human/Mouse Bcl-w Antibody

Immunogen

E. coli-derived recombinant human Bcl-w
Met1-Thr172
Accession # Q92843

Specificity

Detects human and mouse Bcl‑w. Neutralizes recombinant human and mouse Bcl-w, but not recombinant human Bcl‑2 or Bcl‑x.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse Bcl-w Antibody

Bcl-w Inhibition of BID-induced Release of Cytochrome c and Neutralization by BCL-w Antibody.

Bcl-w Inhibition of BID-induced Release of Cytochrome c and Neutralization by BCL-w Antibody.

Cytochrome c released from enriched mouse liver mitochondria solubilized with Triton®X-100 was quantified with the Rat/Mouse Cytochrome c ELISA (Catalog # MCTC0).Untreated: Release of Cytochrome c from untreated mitochondriaBID: Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondriaBID + Bcl-w: Recombinant Human Bcl-w (824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondriaBID + Bcl-w + MAB8241: Mouse Anti-Human/Mouse Bcl-w Monoclonal Antibody (Catalog # MAB8241) neutralized Recombinant Human Bcl-w (824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondria

Applications for Human/Mouse Bcl-w Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize Recombinant Human Bcl-w (Catalog # 824-BW) inhibition of caspase-8-cleaved Recombinant Human BID (Catalog # 882-B8) induced release of cytochrome c from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA Kit (Catalog # MCTC0).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-w

Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

References

  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Kroemer, G. (1997) Nature Med. 3:614.
  3. Ross, J.A., et al. (1998) Nat. Genet. 18:251.
  4. Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
  5. Yan, W. et al. (2000) Mol. Endocrin. 14:682.

Long Name

B Cell Lymphoma/Leukemia w

Alternate Names

BCL2L2, Bclw

Entrez Gene IDs

599 (Human); 12050 (Mouse)

Gene Symbol

BCL2L2

UniProt

Q92843

Additional Bcl-w Products

Product Documents for Human/Mouse Bcl-w Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Bcl-w Antibody

For research use only

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