Human/Mouse Bcl-x Antibody
R&D Systems, part of Bio-Techne | Catalog # AF800
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunoprecipitation, Simple Western, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
KLH-coupled Bcl-x synthetic peptide
CSAINGNPSWHLADSPAVNGA
CSAINGNPSWHLADSPAVNGA
Specificity
Detects human and mouse Bcl‑x in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse Bcl-x Antibody
Detection of Human and Mouse Bcl‑x by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 104cells, lane 1 and 2.5 x 104cells, lane 2) and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl-x at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunoprecipitation of Human Bcl-x.
Bcl-x was immunoprecipitated from lysates (7 x 105cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) for 30‑60 minutes at 4 °C. Bcl-x-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bcl-x was detected by Western blot using 0.3 µg/mL Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). View our recommended buffer recipes for immunoprecipitation.
Detection of Human and Mouse Bcl-x by Simple WesternTM.
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑x at approximately 34 kDa (as indicated) using 3 µg/mL of Rabbit Anti-Human/Mouse Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse Bcl-x Antibody
Application
Recommended Usage
Immunoprecipitation
5 µg/106 cells
Sample: A431 human epithelial carcinoma cell line, see our available Western blot detection antibodies
Sample: A431 human epithelial carcinoma cell line, see our available Western blot detection antibodies
Simple Western
3 µg/mL
Sample: A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Western Blot
0.3 µg/mL
Sample: A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Reviewed Applications
Read 1 review rated 5 using AF800 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-x
Long Name
B Cell Lymphoma/Leukemia x
Alternate Names
BCL2L1, Bclx
Gene Symbol
BCL2L1
Additional Bcl-x Products
Product Documents for Human/Mouse Bcl-x Antibody
Product Specific Notices for Human/Mouse Bcl-x Antibody
For research use only
Loading...
Loading...
Loading...
Loading...