Human/Mouse BID Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB860
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 91508
Product Specifications
Immunogen
E. coli-derived recombinant mouse BID
Met1-Asp195
Accession # P70444
Met1-Asp195
Accession # P70444
Specificity
Detects human and mouse BID in Western blots. Detects full-length mouse BID and the 15 kDa carboxyl-terminal fragment (tBID) generated by cleavage with Caspase-8. The antibody also detects human BID. However, in Western blots of recombinant human and mouse BID, the antibody is most sensitive for mouse BID.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Human/Mouse BID Antibody
Detection of Human and Mouse BID by Western Blot.
Western blot shows lysates of Recombinant Mouse BID and Recombinant Human BID untreated (-) or treated (+) with Caspase-8. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands for BID were detected at approximately 12 kDa and 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Detection of Human and Mouse BID by Western Blot.
Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for BID at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse BID Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse BID and Recombinant Human BID untreated or treated with Caspase-8, SVEC4‑10 mouse vascular endothelial cell line, and Jurkat human acute T cell leukemia cell line
Sample: Recombinant Mouse BID and Recombinant Human BID untreated or treated with Caspase-8, SVEC4‑10 mouse vascular endothelial cell line, and Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using MAB860 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BID
Long Name
BH3 Interacting Domain Death Agonist
Alternate Names
apoptic death agonist, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, desmocollin type 4, FP497, Human BID coding sequence, MGC15319, MGC42355, p22 BID, tbid
Gene Symbol
BID
UniProt
Additional BID Products
Product Documents for Human/Mouse BID Antibody
Product Specific Notices for Human/Mouse BID Antibody
For research use only
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