Human/Mouse/Bovine Insulin APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1417A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine
Cited:
Human, Mouse, Rat
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 182410
Product Specifications
Immunogen
E. coli-derived recombinant human Insulin
Specificity
Detects bovine, human, and mouse insulin.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Bovine Insulin APC-conjugated Antibody
Detection of Insulin in betaTC‑6 Mouse Cell Line by Flow Cytometry.
betaTC-6 mouse beta cell insulinoma cell line was stained with Rat Anti-Human/Mouse/Bovine Insulin APC-conjugated Monoclonal Antibody (Catalog # IC1417A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse/Bovine Insulin APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: betaTC‑6 mouse beta cell insulinoma cell line
Sample: betaTC‑6 mouse beta cell insulinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Insulin
Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.
Alternate Names
IDDM2, ILPR, INS, IRDN, MODY10
Gene Symbol
INS
Additional Insulin Products
Product Documents for Human/Mouse/Bovine Insulin APC-conjugated Antibody
Product Specific Notices for Human/Mouse/Bovine Insulin APC-conjugated Antibody
For research use only
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