Human/Mouse Brachyury APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC2085A
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Brachyury
Ser2-Glu202
Accession # O15178
Ser2-Glu202
Accession # O15178
Specificity
Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Brachyury APC-conjugated Antibody
Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry.
D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085A, filled histogram) or isotype control antibody (IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
Detection of Brachyury in IPSC mesoderm differentiation cells by Flow Cytometry.
IPSC mesoderm differentiation cells treated with IPSC mesoderm differentiation (SC030B) for 2 days were stained with Goat Anti-Human/Mouse Brachyury APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with FC004 and permeabilized with FoxP3 Perm. View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse Brachyury APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and IPSC mesoderm differentiation cells treated with IPSC mesoderm differentiation (Catalog # SC030B) for 2 days.
Sample: D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and IPSC mesoderm differentiation cells treated with IPSC mesoderm differentiation (Catalog # SC030B) for 2 days.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using IC2085A in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Brachyury
Alternate Names
brachyury protein, MGC104817, Protein T, SAVA, T brachyury (mouse) homolog, T brachyury homolog, T Brachyury Transcription Factor, T, brachyury homolog (mouse), T-Box Transcription Factor T, TBXT, TF, TFT
Entrez Gene IDs
6862 (Human)
Gene Symbol
TBXT
UniProt
Additional Brachyury Products
Product Documents for Human/Mouse Brachyury APC-conjugated Antibody
Product Specific Notices for Human/Mouse Brachyury APC-conjugated Antibody
For research use only
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