Human/Mouse Cadherin-17 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8524
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cadherin-17
Glu22-Met786
Accession # Q9R100
Glu22-Met786
Accession # Q9R100
Specificity
Detects mouse and human Cadherin-17 in direct ELISAs and Western blots. In direct ELISAs, less then 10% cross-reactivity with recombinant human Cadherin-17 is observed.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse Cadherin-17 Antibody
Detection of Human and Mouse Cadherin-17 by Western Blot.
Western blot shows lysates of human colon tissue and mouse colon tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse Cadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cadherin-17 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cadherin‑17 in Mouse Intestine.
Cadherin-17 was detected in perfusion fixed frozen sections of mouse intestine using Rabbit Anti-Mouse Cadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) at 1:250 dilution overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane of glandular cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Human and mouse Cadherin‑17 by Simple WesternTM.
Simple Western lane view shows lysates of human colon tissue and mouse colon tissue, loaded at 0.2 mg/mL. Specific bands were detected for Cadherin‑17 at approximately 152 and 146 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human/Mouse Cadherin‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse Cadherin-17 Antibody
Application
Recommended Usage
Immunohistochemistry
1:250 dilution
Sample: Perfusion fixed frozen sections of mouse intestine
Sample: Perfusion fixed frozen sections of mouse intestine
Simple Western
1:100 dilution
Sample: Human colon tissue and mouse colon tissue
Sample: Human colon tissue and mouse colon tissue
Western Blot
1:1000 dilution
Sample: Human colon tissue and mouse colon tissue
Sample: Human colon tissue and mouse colon tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied as a 0.2 μm filtered solution in PBS containing BSA, Glycerol and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Cadherin-17
Alternate Names
Cadherin17, CDH17
Gene Symbol
CDH17
UniProt
Additional Cadherin-17 Products
Product Documents for Human/Mouse Cadherin-17 Antibody
Product Specific Notices for Human/Mouse Cadherin-17 Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
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