Human/Mouse CBL Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5998
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CBL
Asp775-Thr906
Accession # P22681
Asp775-Thr906
Accession # P22681
Specificity
Detects human and mouse CBL in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse CBL Antibody
Detection of Human CBL by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse CBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5998) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CBL at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human/Mouse CBL by Simple WesternTM.
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cells, K562 human chronic myelogenous leukemia cells, and Raji human Burkitt's lymphoma cells, loaded at 0.2 mg/mL. A specific band was detected for CBL at approximately 116 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse CBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5998). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse CBL Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Daudi human Burkitt's lymphoma cells, K562 human chronic myelogenous leukemia cells, Raji human Burkitt's lymphoma cells
Sample: Daudi human Burkitt's lymphoma cells, K562 human chronic myelogenous leukemia cells, Raji human Burkitt's lymphoma cells
Western Blot
0.5 µg/mL
Sample: MCF‑7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and Raji human Burkitt's lymphoma cell line
Sample: MCF‑7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and Raji human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CBL
CBL (Lake Casitas, California mouse B-lineage Lymphoma; also RING finger protein 55) is a 120‑125 kDa cytosolic member of the CBL family of proteins. It is widely expressed, being found in cells such as fibroblasts, adipocytes, osteoclasts, podocytes and mammary epithelium. CBL negatively regulates RTK signaling by acting as an E3 ubiquitin ligase. Following activation via phosphorylation, CBL will interact with multiple signaling molecules such as Src, ZAP-70, EGFR and SHP1. Human CBL is 906 amino acids (aa) in length. It contains an N-terminal phosphoTyr-binding region that consists of one alpha-helix bundle, an EF hand segment, and an SH2 domain (aa 47‑342), a RING finger domain that interacts with E2 enzymes (aa 381‑423) a Pro-rich SH3 domain (aa 477‑688), and a Leu‑zipper/Ubiquitin‑associated region (aa 861‑892). There is one potential alternative start site at Met55. Over aa 775‑906, human CBL shares 91% aa identity with mouse CBL.
Long Name
Casitas B-lineage Lymphoma, E3 Ubiquitin Protein Ligase CBL
Alternate Names
CBL2, RNF55
Gene Symbol
CBL
UniProt
Additional CBL Products
Product Documents for Human/Mouse CBL Antibody
Product Specific Notices for Human/Mouse CBL Antibody
For research use only
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