Human/Mouse CX3CR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5825
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, In vivo assay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CX3CR1
Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274
Accession # Q9Z0D9
Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274
Accession # Q9Z0D9
Specificity
Detects human and mouse CX3CR1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse CX3CR1 Antibody
Detection of Human and Mouse CX3CR1 by Western Blot.
Western blot shows lysates of M1 mouse myeloid leukemia cell line and NK-92 human natural killer lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CX3CR1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CX3CR1 in M1 Mouse Cell Line by Flow Cytometry.
M1 mouse myeloid leukemia cell line was stained with Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).Applications for Human/Mouse CX3CR1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: M1 mouse myeloid leukemia cell line
Sample: M1 mouse myeloid leukemia cell line
Western Blot
1 µg/mL
Sample: M1 mouse myeloid leukemia cell line and NK‑92 human natural killer lymphoma cell line
Sample: M1 mouse myeloid leukemia cell line and NK‑92 human natural killer lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using AF5825 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CX3CR1
Long Name
CX3C Chemokine Receptor 1
Alternate Names
CMKbRL1, V28
Gene Symbol
CX3CR1
UniProt
Additional CX3CR1 Products
Product Documents for Human/Mouse CX3CR1 Antibody
Product Specific Notices for Human/Mouse CX3CR1 Antibody
For research use only
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