Human/Mouse Cyclin A2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5999
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse, Xenograft
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Cyclin A2
Ala73-Tyr199
Accession # P20248
Ala73-Tyr199
Accession # P20248
Specificity
Detects human and mouse Cyclin A2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Cyclin A2 Antibody
Detection of Human and Mouse Cyclin A2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Cyclin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclin A2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse Cyclin A2 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclin A2
Cyclin A2 (also CCNA2) is a member of the cyclin AB subfamily, cyclin family of proteins. Cyclin A2 is ubiquitously expressed, in contrast to CyclinA1 that only appears in late pachytene spermatocytes. It associates with CDK1 during G2 and M phase, and CDK2 in S phase, of the cell cycle. Binding to SCAPER restricts Cyclin A2 to the cytoplasm. In both compartments, it provides substrate specificity to a phosphorylating complex that promotes entry into, and progression through, mitosis. Human Cyclin A2 is 432 amino acids (aa) in length. It contains two cyclin box folds (aa 210‑299 and 309‑430) and four acetylation sites that induce molecule turnover (Lys54/68/95/112). Over aa 73‑199, human Cyclin A2 shares 83% aa identity with mouse Cyclin A2.
Alternate Names
CCNA, CCNA2
Gene Symbol
CCNA2
UniProt
Additional Cyclin A2 Products
Product Documents for Human/Mouse Cyclin A2 Antibody
Product Specific Notices for Human/Mouse Cyclin A2 Antibody
For research use only
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