Human/Mouse Cyclin D1/D2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4196
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Cyclin D1
Met1-Ile295
Accession # P24385
Met1-Ile295
Accession # P24385
Specificity
Detects human and mouse Cyclin D1/D2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Cyclin D1/D2 Antibody
Detection of Human/Mouse Cyclin D1/D2 by Western Blot.
Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, U2OS human osteosarcoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse Cyclin D1/D2 Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human Cyclin D1, D2, and D3 (5 ng/lane) were included. A specific band for Cyclin D1 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Cyclin D1/D2 in MCF‑7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line was stained with Goat Anti-Human/Mouse Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4196, filled histogram) or isotype control AA175antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldeyde and permeabilized with saponin.Applications for Human/Mouse Cyclin D1/D2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample:
Sample:
MCF‑7 human breast cancer cell line fixed with paraformaldeyde and permeabilized with saponin.
Western Blot
1 µg/mL
Sample: Neuro-2A mouse neuroblastoma cell line, U2OS human osteosarcoma cell line, and MCF-7 human breast cancer cell line
Sample: Neuro-2A mouse neuroblastoma cell line, U2OS human osteosarcoma cell line, and MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclin D1/D2
The D-type cyclins (cyclins D1, D2, and D3) and their associated kinases, Cdk4 and Cdk6, play an important role in the progression from G0/G1 to S phase in the mammalian cell cycle. Cyclin D complexes containing Cdk4 or Cdk6 phosphorylate the retinoblastoma protein (pRb). pRb phosphorylation leads to the release of E2F transcription factors, which activate the expression of S-phase genes and thereby induce cell cycle progression. Cyclin D1, independent of Cdk4 activity, functions as a transcriptional modulator by regulating the activity of several transcription factors, such as estrogen receptor, Myb, and STAT3. Cyclin D1 has also been linked to the development and progression of several cancers including breast, bladder, esophagus, and lung. Overexpression of Cyclin D2 has been reported in gastric carcinoma, ovarian granulose cell tumors, and hematopoietic cell cancers, while in breast cancers, cyclin D2 expression is undetectable in 80% of the tumors.
Alternate Names
BCL1;CCND1;D11S287E;G1/S-specific cyclin-D1;PRAD1;U21B31
Gene Symbol
CCND1
UniProt
Additional Cyclin D1/D2 Products
Product Documents for Human/Mouse Cyclin D1/D2 Antibody
Product Specific Notices for Human/Mouse Cyclin D1/D2 Antibody
For research use only
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