Human/Mouse Fatty Acid Synthase/FASN Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5927
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse
Applications
Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 647113
Product Specifications
Immunogen
E. coli-derived recombinant human Fatty Acid Synthase/FASN
Ser10-Cys212
Accession # P49327
Ser10-Cys212
Accession # P49327
Specificity
Detects human and mouse Fatty Acid Synthase/FASN in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse Fatty Acid Synthase/FASN Antibody
Detection of Human and Mouse Fatty Acid Synthase/FASN by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Fatty Acid Synthase/FASN Monoclonal Antibody (Catalog # MAB5927) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Fatty Acid Synthase/FASN at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western Blot Shows Human Fatty Acid Synthase/FASN Specificity by Using Knockout Cell Line.
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Fatty Acid Synthase/FASN knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Fatty Acid Synthase/FASN Monoclonal Antibody (Catalog # MAB5927) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Fatty Acid Synthase/FASN at approximately 275 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Fatty Acid Synthase/FASN by Simple WesternTM.
Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Fatty Acid Synthase/FASN at approximately 213 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Fatty Acid Synthase/FASN Monoclonal Antibody (Catalog # MAB5927). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.Applications for Human/Mouse Fatty Acid Synthase/FASN Antibody
Application
Recommended Usage
Knockout Validated
Fatty
Acid Synthase/FASN is specifically detected in HEK293T human embryonic kidney parental cell line but is
not detectable in Fatty Acid Synthase/FASN knockout HEK293T cell line.
Simple Western
20 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and mouse liver tissue
Sample: HepG2 human hepatocellular carcinoma cell line and mouse liver tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fatty Acid Synthase/FASN
FASN (Fatty Acid Synthase; also FAS) is a cytosolic 270-280 kDa member of the short chain dehydrogenase/reductase family 27X. It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes, and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA, and likely mediates the transfer of fatty acids to peptides. Human FASN is 2511 amino acids (aa) in length ands contains multiple domains, including a beta-ketoacyl synthase domain (aa 1-414), an acyl and malonyl transferase domain (aa 429-817), an enoyl reductase domain (aa 1563-1863), a beta-ketoacyl reductase domain (aa 1864-2118) and a thioesterase domain (aa 2207‑2511). FASN exists as an antiparallel homodimer. There is one theoretical alternative start site at Met2073. Over aa 9-212, human FASN shares 92% aa identity with mouse FASN.
Alternate Names
FASN, OA-519, SDR27X1
Gene Symbol
FASN
UniProt
Additional Fatty Acid Synthase/FASN Products
Product Documents for Human/Mouse Fatty Acid Synthase/FASN Antibody
Product Specific Notices for Human/Mouse Fatty Acid Synthase/FASN Antibody
For research use only
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