Detects mouse Frizzled-1 in direct ELISAs and Western blots and detects human Frizzled-1 by flow cytometry. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human/Mouse Frizzled-1 Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Western Blot

1 µg/mL
Sample: Recombinant Human Frizzled-1 Fc Chimera
Recombinant Mouse Frizzled-1 Fc Chimera (Catalog # 1120-FZ)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-1

Frizzled-1 belongs to the frizzled family of 7-transmembrane domain proteins that are receptors for Wnt proteins. Frizzled-1 is expressed in a wide variety of tissues and is involved in cell differentiation, embryonal development and tumorigenesis.

Alternate Names

Frizzled1, FZD1

Entrez Gene IDs

8321 (Human); 14362 (Mouse)

Gene Symbol

FZD1

UniProt

O70421

Additional Frizzled-1 Products

Product Documents for Human/Mouse Frizzled-1 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Frizzled-1 Biotinylated Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Mouse Frizzled-1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM11201

Key Product Details

Species Reactivity

Validated:

Cited:

Applications