Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -4, -7, or -8 is observed. By immunohistochemistry, this antibody also detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. This antibody also detects Frizzled-3 on human HEK293 cells by immunocytochemistry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Human/Mouse Frizzled-3 Antibody

Detection of Frizzled-3 in Mouse Cortex.

Frizzled-3 was detected in immersion fixed paraffin-embedded sections of mouse cortex using Rat Anti-Human/Mouse Frizzled-3 Monoclonal Antibody (Catalog # MAB1001) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005) . Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Frizzled-3 in Human Cortex.

Frizzled-3 was detected in immersion fixed paraffin-embedded sections of human cortex using Rat Anti-Human/Mouse Frizzled-3 Monoclonal Antibody (Catalog # MAB1001) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005) . Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse Frizzled-3 Antibody

Application

Recommended Usage

Immunohistochemistry

1-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human cortex and mouse cortex.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-3

Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.

Alternate Names

Frizzled3, FZD3

Entrez Gene IDs

7976 (Human); 14365 (Mouse)

Gene Symbol

FZD3

UniProt

Q61086

Additional Frizzled-3 Products

Product Documents for Human/Mouse Frizzled-3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Frizzled-3 Antibody

For research use only

Citations
Reviews