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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # 169310

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
Met1-Val157
Accession # Q61086

Specificity

Detects mouse Frizzled-3 in direct ELISAs and Western blots. In immunohistochemistry detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and on human HEK 293 cells. In direct ELISAs, no cross‑reactivity with Frizzled-1, -4, -7 or -8 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Frizzled-3 Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13-15)

Western Blot

1 µg/mL
Sample: Recombinant Mouse Frizzled-3

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-3

Frizzled-3 is a member of the Frizzled family of G-protein coupled receptors that have seven transmembrane domains and an extracellular CRD. Frizzled proteins function as receptors for Wnt proteins.

Alternate Names

Frizzled3, FZD3

Entrez Gene IDs

7976 (Human); 14365 (Mouse)

Gene Symbol

FZD3

UniProt

Additional Frizzled-3 Products

Product Documents for Mouse Frizzled-3 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Frizzled-3 Biotinylated Antibody

For research use only

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