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Human/Mouse GDPD2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7026

R&D Systems, part of Bio-Techne
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MAB7026
MAB7026-SP

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 501016

Product Summary for Human/Mouse GDPD2 Antibody

Immunogen

HEK293 human embryonic kidney cell line transfected with human GDPD2
Accession # Q9HCC8

Specificity

Detects human and mouse GDPD2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse GDPD2 Antibody

GDPD2 antibody in MC3T3-E1 Mouse Cell Line by Immunocytochemistry (ICC).

GDPD2 in MC3T3‑E1 Mouse Cell Line.

GDPD2 was detected in immersion fixed MC3T3-E1 mouse preosteoblast cell line using Mouse Anti-Human GDPD2 Monoclonal Antibody (Catalog # MAB7026) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse GDPD2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MC3T3‑E1 mouse preosteoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB7026 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDPD2

Glycerophosphodiester phosphodiesterase 3 (GDPD2), also known as GDE3, is a 65 kDa 7TM cell surface protein that is highly expressed in bone and spleen. GDPD2 promotes osteoblast differentiation and induces changes in the actin cytoskeleton. Its 225 amino acid (aa) N-terminal ECD contains a domain that is homologous to bacterial glycerophosphodiester phosphodiesterases. Human GDPD2 shares 85% aa sequence identity with mouse and rat GDPD2.

Long Name

Glycerophosphodiester Phosphodiesterase Domain Containing 2

Alternate Names

GDE3, OBDPF

Entrez Gene IDs

54857 (Human); 71584 (Mouse); 302421 (Rat)

Gene Symbol

GDPD2

UniProt

Additional GDPD2 Products

Product Documents for Human/Mouse GDPD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse GDPD2 Antibody

For research use only

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