Human/Mouse Glucokinase/GCK Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7840
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 849520
Product Specifications
Immunogen
E. coli-derived recombinant human Glucokinase/GCK
Val16-Gln465
Accession # P35557
Val16-Gln465
Accession # P35557
Specificity
Detects human Glucokinase/GCK in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PRF-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse Glucokinase/GCK Antibody
Detection of Human and Mouse Glucokinase/GCK by Western Blot.
Western blot shows lysates of Huh-7 human hepatoma cell line, Hepa 1-6 mouse hepatoma cell line, and HT-2 mouse T cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Glucokinase/GCK Monoclonal Antibody (Catalog # MAB7840) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glucokinase/GCK at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse Glucokinase/GCK Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Huh‑7 human hepatoma cell line, Hepa 1‑6 mouse hepatoma cell line, and HT‑2 mouse T cell line
Sample: Huh‑7 human hepatoma cell line, Hepa 1‑6 mouse hepatoma cell line, and HT‑2 mouse T cell line
Reviewed Applications
Read 1 review rated 4 using MAB7840 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glucokinase/GCK
References
- Aleshin, A.E. et al.(1998) Structure 6:39.
- Takeda, J. et al. (1993) J. Biol. Chem. 268:15200.
- Lange, A.J. et al. (1991) Biochem. J. 277:159.
- Wu, Z.L. (2011) PLoS ONE 6(8):e23172.
- Xu, L.Z. et al. (1995) J. Biol. Chem. 270:9939.
- Gidh-Jain, M. et al. (1993) Proc. Natl. Acad. Sci. U. S. A. 90:1932.
- Stoffel, M. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:7698.
- Hattersley, A.T. et al. (1992) Lancet 339:1307.
- Gloyn, A.L. et al. (2003) Diabetes 52:2433.
Long Name
Glucose Kinase
Alternate Names
FGQTL3, GCK, GLK, Hexokinase-4, Hexokinase-D, HHF3, HK4, HXKP, LGLK
Gene Symbol
GCK
UniProt
Additional Glucokinase/GCK Products
Product Documents for Human/Mouse Glucokinase/GCK Antibody
Product Specific Notices for Human/Mouse Glucokinase/GCK Antibody
For research use only
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