Human/Mouse GRAP2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4640
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GRAP2
Met1-Arg330
Accession # O75791
Met1-Arg330
Accession # O75791
Specificity
Detects endogenous human and mouse GRAP2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse GRAP2 Antibody
Detection of Human/Mouse GRAP2 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse GRAP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4640) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRAP2 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse GRAP2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and CTLL-2 mouse cytotoxic T cell line
Sample: K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and CTLL-2 mouse cytotoxic T cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRAP2
GRAP2 (GRB2 related adaptor protein 2), a divergent member of the GRB2/Sem5/Drk family, is an adaptor protein involved in leukocyte-specific protein-tyrosine kinase signaling. GRAP2 shares similar structural organization as GRB2 and GRAP with a central SH2 domain flanked by 2 SH3 domains. However, unlike GRB2 and GRAP, the SH2 domain and the C-terminal SH3 domain of GRAP2 contains a unique 120 amino acid glutamine/proline rich sequence. In T lymphocytes, GRAP2 interacts with LAT and SLP-76 to regulate NF-AT (nuclear factor of activated T cells) and form a signaling complex with HPK1 to mediate the JNK signaling pathway. GRAP2 also interacts with M-CSFR and the activated T cell co-stimulatory receptor CD28.
Long Name
GRB2-related Adaptor Protein 2
Alternate Names
GADS, GRB2L, GRBLG, GRBX, Grf40, GRID, GRPL, Mona
Gene Symbol
GRAP2
UniProt
Additional GRAP2 Products
Product Documents for Human/Mouse GRAP2 Antibody
Product Specific Notices for Human/Mouse GRAP2 Antibody
For research use only
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