Human/Mouse Histone Deacetylase 8/
HDAC8 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF4359
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Histone Deacetylase 8/HDAC8 isoform 1
Met1-Val377
Accession # ACT64512
Met1-Val377
Accession # ACT64512
Specificity
Detects human Histone Deacetylase 8/HDAC8 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human/Mouse Histone Deacetylase 8/HDAC8 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Histone Deacetylase 8/HDAC8 (Catalog # 4359-DA)
Sample: Recombinant Human Histone Deacetylase 8/HDAC8 (Catalog # 4359-DA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Histone Deacetylase 8/HDAC8
Human Histone Deacetylase 8 (HDAC8) is a member of the class I Histone Deacetylases (HDACs) (1, 2). HDACs are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells (3). HDACs catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer (4). Expression of HDAC8 is restricted to cells showing smooth muscle differentiation in normal human tissue and is a novel marker of smooth muscle differentiation (5, 6). Like other class I and II HDAC members, the activity of HDAC8 is sensitive to HDAC inhibitor trichostatin A (1).
References
- Hu, E. et al. (2000) J. Biol. Chem. 275:15254.
- Annemieke, J et al. (2003) Biochem. J. 370:737.
- Gray, S. and T. Ekstrom (2001) Exp. Cell Res. 262:75.
- Yang, X. and S. Gregoire (2005) Mol. Cel. Biol. 25:2873.
- Waltregny, D. et al. (2004) Am. J. Pathol. 165:553.
- De Levak, L. et al. (2006) Am. J. Surg. Pathol. 30:319.
Alternate Names
HDAC8, HDACL1, RPD3
Gene Symbol
HDAC8
UniProt
Additional Histone Deacetylase 8/HDAC8 Products
Product Specific Notices for Human/Mouse Histone Deacetylase 8/HDAC8 Biotinylated Antibody
For research use only
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