Human/Mouse HO-2/HMOX2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3170
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 322913
Product Specifications
Immunogen
E. coli-derived recombinant human HO‑2/HMOX2
Met1-Met316
Accession # P30519
Met1-Met316
Accession # P30519
Specificity
Detects human and mouse HO‑2/HMOX2 in Western blots. Endogenous rat HO-2 is detectable but has a higher background. In Western blots, no cross-reactivity with recombinant human HO-1/HMOX1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse HO-2/HMOX2 Antibody
Detection of Human and Mouse HO‑2/HMOX2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse HO-2/HMOX2 Monoclonal Antibody (Catalog # MAB3170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO-2/HMOX2 was detected at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse HO-2/HMOX2 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and DA3 mouse myeloma cell line
Sample: HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and DA3 mouse myeloma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HO-2/HMOX2
Heme Oxygenase 2 (HO-2), also known as HMOX2, is a 36 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme Oxygenase occurs as 2 isozymes, the constitutively expressed Heme Oxygenase-2(HO-2/HMOX2) and the inducible Heme Oxygenase-1 (HO-1/HMOX1). HO-1 expression is induced by heme and other non-heme compounds. Human HO-2 shares 42% amino acid sequence identity with human HO-1 and 89% amino acid sequence identity with mouse and rat HO-2.
Long Name
Heme Oxygenase 2
Alternate Names
HMOX2, HO2
Gene Symbol
HMOX2
UniProt
Additional HO-2/HMOX2 Products
Product Documents for Human/Mouse HO-2/HMOX2 Antibody
Product Specific Notices for Human/Mouse HO-2/HMOX2 Antibody
For research use only
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