Human/Mouse IL-5 Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC405G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Intracellular Staining by Flow Cytometry
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Rat IgG1 Clone # TRFK5
Product Specifications
Immunogen
Mouse T cell clone-derived partially purified recombinant mouse IL-5
Specificity
Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Human/Mouse IL-5 Alexa Fluor® 488-conjugated Antibody
Detection of CCL3/MIP-1 alpha in Raw264.7 cells treated with 1ug/ml LPS and 3uM Monensin overnight by Flow Cytometry
Raw264.7 cells treated with 1 μg/ml LPS and 3uM Monensin overnight were stained with Rat Anti-Mouse CCL3/MIP-1 alpha Alexa Fluor 488-conjugated Monoclonal Antibody (Catalog # IC450G, filled histogram) or , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse IL-5 Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Sample: - Human peripheral blood monocytes treated with PMA and Calcium Ionomycin, fixed with paraformaldehyde and permeabilized with saponin
- Raw264.7 cells treated with 1 μg/ml LPS and 3uM Monensin overnight
Sample: - Human peripheral blood monocytes treated with PMA and Calcium Ionomycin, fixed with paraformaldehyde and permeabilized with saponin
- Raw264.7 cells treated with 1 μg/ml LPS and 3uM Monensin overnight
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months, 2 to 8 °C under sterile conditions after opening.
Background: IL-5
IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.
Long Name
Interleukin 5
Alternate Names
BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF
Entrez Gene IDs
Gene Symbol
IL5
Additional IL-5 Products
Product Specific Notices for Human/Mouse IL-5 Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...