Human/Mouse Jumonji/JARID2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6090
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
CyTof, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Jumonji/JARID2
Met1-Thr159
Accession # Q92833
Met1-Thr159
Accession # Q92833
Specificity
Detects human and mouse Jumonji/JARID2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human JARID2B is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse Jumonji/JARID2 Antibody
Detection of Human Jumonji/JARID2 by Western Blot.
Western blot shows lysates of IMR-32 human neuroblastoma cell line and Neuro-2A mouse neuroblastoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse Jumonji/JARID2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6090) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Jumonji/JARID2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human/Mouse Jumonji/JARID2 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: IMR‑32 human neuroblastoma cell line and Neuro‑2A mouse neuroblastoma cell line
Sample: IMR‑32 human neuroblastoma cell line and Neuro‑2A mouse neuroblastoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jumonji/JARID2
Jumonji [Japanese for "cruciform/cross" also JARID2 (Jumonji/ARID domain‑containing protein 2) and JMJ] is a 140‑155 kDa member of the JMJ family of proteins. It is a nuclear complex coordinator that interacts with the G9a and GLP methyltransferases to repress gene transcription. Jumonji is expressed in mammalian embryonic neurons, megakaryocytes, hepatocytes and cardiomyocytes. Human Jumonji is 1246 amino acids (aa) in length. It contains an NLS (aa 1‑131), a transcriptional repressor domain (aa 132‑222), a DNA binding region (aa 558‑709) and a nonfunctional histone demethylase domain (aa 916‑1042). There are potential splice variants. One shows an alternative start site at Met173, another shows a deletion of aa 304‑1151, and a third contains a 22 aa substitution for aa 1‑60, coupled to a 50 aa substitution for aa 949‑1246. Over aa 1‑159, human Jumonji shares 96% aa identity with mouse Jumonji.
Alternate Names
JARID2, JMJ
Gene Symbol
JARID2
UniProt
Additional Jumonji/JARID2 Products
Product Documents for Human/Mouse Jumonji/JARID2 Antibody
Product Specific Notices for Human/Mouse Jumonji/JARID2 Antibody
For research use only
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