Human/Mouse JunD Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5526
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 512709
Product Specifications
Immunogen
E. coli-derived recombinant human JunD
Lys47-Gly156
Accession # CAA40010
Lys47-Gly156
Accession # CAA40010
Specificity
Detects endogenous human and mouse JunD in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse JunD Antibody
Detection of Human/Mouse JunD by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse JunD Monoclonal Antibody (Catalog # MAB5526) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for JunD at approximately 40 kDa and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse JunD Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: JunD
JunD is a member of the Jun family of nuclear transcription factors. It dimerizes with other Jun or Fos family members to form the AP1 transcription factor. Unlike c‑Jun or JunB, JunD antagonizes Ras transformation and accumulates as cells enter quiescence. JunD is a 347 amino acid (aa), 39 kDa protein (JunD-FL) that has a 303 aa, 34 kDa isoform ( deltaJunD) which begins at aa 44. Over the sequence used as an immunogen, human JunD shares 72% aa identity with mouse and rat JunD.
Long Name
JunD Proto-oncogene
Alternate Names
AP-1
Gene Symbol
JUND
UniProt
Additional JunD Products
Product Documents for Human/Mouse JunD Antibody
Product Specific Notices for Human/Mouse JunD Antibody
For research use only
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