Human/Mouse Leukotriene A4 Hydrolase/
LTA4H Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4008
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Leukotriene A4 Hydrolase/LTA4H
Pro2-Asp611
Accession # P09960
Pro2-Asp611
Accession # P09960
Specificity
Detects human and mouse Leukotriene A4 Hydrolase/LTA4H in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse Leukotriene A4 Hydrolase/LTA4H Antibody
Detection of Human and Mouse Leukotriene A4 Hydrolase/LTA4H by Western Blot.
Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, NCI-N87 human gastric carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse Leukotriene A4 Hydrolase/LTA4H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4008) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Leukotriene A4 Hydrolase/LTA4H at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human/Mouse Leukotriene A4 Hydrolase/LTA4H Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: NTera‑2 human testicular embryonic carcinoma cell line, NCI‑N87 human gastric carcinoma cell line, and C2C12 mouse myoblast cell line
Sample: NTera‑2 human testicular embryonic carcinoma cell line, NCI‑N87 human gastric carcinoma cell line, and C2C12 mouse myoblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Leukotriene A4 Hydrolase/LTA4H
Leukotrienes are a family of lipid mediators important in a variety of allergic and inflammatory reactions. Synthesized by leukocytes, these molecules are divided into two classes, the spasmogenic cysteinyl leukotrienes and LTB4, a classical chemoattractant (1). Encoded by the LTA4H gene, Leukotriene A4 Hydrolase catalyzes the conversion of unstable epoxide LTA4 to LTB4, which is the final and committed step in LTB4 biosynthesis. As a bifunctional zinc metalloenzyme, LTA4H also acts as an arginyl aminopeptidase (2). LTA4H is a drug target for anti-inflammation, and for cancer prevention and therapy (1, 3).
The mature chain of human LTA4H consists of 610 amino acids (residues 2‑611). It is highly specific for LTA4, which also covalently modifies and inhibits the enzyme. The aminopeptidase activity is enhanced by monovalent anions (1). R&D Systems rhLTA4H corresponds to the mature chain, and is characterized by its arginyl aminopeptidase activity.
References
- Haeggstrom, J.Z. (2006) J. Biol. Chem. 279:50639.
- Orning, L. et al. (1994) J. Biol. Chem. 269:11269.
- Chen, X. et al. (2004) Curr. Cancer Drug Targets 4:267.
Alternate Names
LTA4H
Gene Symbol
LTA4H
UniProt
Additional Leukotriene A4 Hydrolase/LTA4H Products
Product Documents for Human/Mouse Leukotriene A4 Hydrolase/LTA4H Antibody
Product Specific Notices for Human/Mouse Leukotriene A4 Hydrolase/LTA4H Antibody
For research use only
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