Human/Mouse MSK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2518
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Rat, Transgenic Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MSK1
Met1-Ala802
Accession # O75582
Met1-Ala802
Accession # O75582
Specificity
Detects human and mouse MSK1 in Western blots. This antibody does not detect recombinant human MSK2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse MSK1 Antibody
Detection of Human/Mouse MSK1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse MSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2518) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human MSK1 and MSK2 (2 ng/lane) were included. A specific band for MSK1 was detected at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MSK1 in HeLa Human Cell Line.
MSK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse MSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2518) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse MSK1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and TS1 mouse helper T cell line
Sample: HeLa human cervical epithelial carcinoma cell line and TS1 mouse helper T cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MSK1
Long Name
Mitogen- and Stress-activated Protein Kinase 1
Alternate Names
RPS6KA5
Gene Symbol
RPS6KA5
UniProt
Additional MSK1 Products
Product Documents for Human/Mouse MSK1 Antibody
Product Specific Notices for Human/Mouse MSK1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...