Human/Mouse Neprilysin/CD10 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1126
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 200103
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Neprilysin/CD10
Tyr52-Trp750
Accession # Q61391
Tyr52-Trp750
Accession # Q61391
Specificity
Detects human and mouse Neprilysin/CD10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human ECE-2 or recombinant mouse Kell is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human/Mouse Neprilysin/CD10 Antibody
Neprilysin/CD10 in Human Kidney.
Neprilysin/CD10 was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Neprilysin/CD10 Monoclonal Antibody (Catalog # MAB1126) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules and glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human and Mouse Neprilysin/CD10 by Western Blot.
Western blot shows lysates of LNCaP human prostate cancer cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Neprilysin/CD10 Monoclonal Antibody (Catalog # MAB1126) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Mouse Neprilysin (Catalog # 1126-ZN) (5 and 50 ng/lane) and Recombinant Human Neprilysin (CHO-expressed) (Catalog # 1182-ZNC) (5 and 50 ng/lane) were included. Specific bands were detected for Neprilysin/CD10 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse Neprilysin/CD10 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of mouse brain (hippocampus) and spinal cord, and immersion fixed paraffin-embedded sections of human kidney
Sample: Perfusion fixed paraffin-embedded sections of mouse brain (hippocampus) and spinal cord, and immersion fixed paraffin-embedded sections of human kidney
Western Blot
1 µg/mL
Sample: LNCaP human prostate cancer cell line, PC‑3 human prostate cancer cell line, Recombinant Human Neprilysin (CHO-expressed) (Catalog # 1182-ZNC), and Recombinant Mouse Neprilysin (Catalog # 1126-ZN)
Sample: LNCaP human prostate cancer cell line, PC‑3 human prostate cancer cell line, Recombinant Human Neprilysin (CHO-expressed) (Catalog # 1182-ZNC), and Recombinant Mouse Neprilysin (Catalog # 1126-ZN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB1126 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neprilysin/CD10
Alternate Names
CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1
Gene Symbol
MME
UniProt
Additional Neprilysin/CD10 Products
Product Documents for Human/Mouse Neprilysin/CD10 Antibody
Product Specific Notices for Human/Mouse Neprilysin/CD10 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...