Human/Mouse NeuroD1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2746
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Rat, Transgenic Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NeuroD1
Ser154-Asp356
Accession # Q13562
Ser154-Asp356
Accession # Q13562
Specificity
Detects NeuroD1 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human NeuroD2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse NeuroD1 Antibody
NeuroD1 in betaTC-6 Mouse Cell Line.
Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed betaTC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse NeuroD1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed beta TC-6 mouse beta cell insulinoma cell line
Sample: Immersion fixed beta TC-6 mouse beta cell insulinoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human NeuroD1
Sample: Recombinant Human NeuroD1
Reviewed Applications
Read 1 review rated 5 using AF2746 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NeuroD1
Long Name
Neurogenic Differentiation Factor 1
Alternate Names
BETA2, BHF-1, NIDDM
Entrez Gene IDs
4760 (Human)
Gene Symbol
NEUROD1
UniProt
Additional NeuroD1 Products
Product Documents for Human/Mouse NeuroD1 Antibody
Product Specific Notices for Human/Mouse NeuroD1 Antibody
For research use only
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