Western blot shows lysates ofSf21S. frugiperdainsect ovarian cell line either mock transfected or transfected with human nNOS, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse nNOS Monoclonal Antibody (Catalog # MAB24161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for nNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

nNOS in Neuro‑2A Mouse Cell Line.

nNOS was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Mouse Anti-Human/Mouse nNOS Monoclonal Antibody (Catalog # MAB24161) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei, cytoplasm and cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of nNOS in Neuro‑2A Mouse Cell Line by Flow Cytometry.

Neuro-2A mouse neuroblastoma cell line was stained with Mouse Anti-Human/Mouse nNOS Monoclonal Antibody (Catalog # MAB24161, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse nNOS Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Neuro-2A mouse neuroblastoma cell line

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Neuro‑2A mouse neuroblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

1 µg/mL
Sample: Sf 21 S. frugiperda insect ovarian cell line transfected with human nNOS and mouse brain tissue

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: nNOS

nNOS is one of three NOS enzymes that catalyze the oxidation of L-argine to L-citruline and nitric oxide. nNOS exists as homodimers containing a cytochrome P450‑like prosthetic heme group in the N-terminal half. It also has a tightly bound FAD and FMN group in the C-terminal half. At least 4 isoforms of human nNOS are known. Human nNOS shares about 55% amino acid sequence identity with eNOS and iNOS. It also shares 96% sequence identity with mouse or rat nNOS.

Long Name

Neuronal Nitric Oxide Synthase

Alternate Names

NOS1

Entrez Gene IDs

4842 (Human); 18125 (Mouse)

Gene Symbol

NOS1

UniProt

P29475

Additional nNOS Products

Product Documents for Human/Mouse nNOS Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse nNOS Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Mouse nNOS Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB24161

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all nNOS Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse nNOS Antibody

Detection of Human and Mouse nNOS by Western Blot.