Human/Mouse Oct-3/4 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1759P
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Rat
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry, Western Blot
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 240408
Product Specifications
Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Met1-Asn265 (Met262Leu)
Accession # Q01860
Specificity
Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human/Mouse Oct-3/4 PE-conjugated Antibody
Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry.
BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 PE-conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Oct‑3/4 in Mouse Stem Cells by Flow Cytometry.
Mouse embryonic stem cells treated with 5 µM Retinoic Acid for 3 days were stained with Rat Anti-Human/Mouse Oct-3/4 PE-conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse Oct-3/4 PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: BG01V human embryonic stem cells and mouse embryonic stem cells treated with Retinoic Acid were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: BG01V human embryonic stem cells and mouse embryonic stem cells treated with Retinoic Acid were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Oct-3/4
Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells (1- 3). Oct-3/4 is required to sustain stem cell self-renewal and pluripotency (4). It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.
References
-
Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
-
Scholer, H.R. et al. (1989) EMBO J. 8:2543.
-
Rosner, M.H. et al. (1990) Nature 345:686.
- Niwa, H. et al. (2000) Nat. Genet. 24:372.
Long Name
Octamer-binding Protein 3/4
Alternate Names
3-Oct, Oct4, Otf3g, Otf4, Pou5f1
Gene Symbol
POU5F1
UniProt
Additional Oct-3/4 Products
Product Documents for Human/Mouse Oct-3/4 PE-conjugated Antibody
Product Specific Notices for Human/Mouse Oct-3/4 PE-conjugated Antibody
For research use only
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