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Human/Mouse Olig2 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF2418

R&D Systems, part of Bio-Techne
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BAF2418

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry, Immunohistochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human Olig2
Met1-Lys323
Accession # Q13516

Specificity

Detects Olig2 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Olig1 and rhOlig3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse Olig2 Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse spinal cord (E11)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Olig2
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olig2

Olig1 and Olig2 are basic-helix-loop-helix (bHLH) transcription factors expressed in the motor neuron progenitor (pMN) domain of the spinal cord that generates motor neurons and oligodendrocytes. Olig1 is involved in the development and maturation of oligodendrocytes. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord.

Long Name

Oligodendrocyte Lineage Transcription Factor 2

Alternate Names

BHLHB1, PRKCBP2, RACK17

Entrez Gene IDs

10215 (Human)

Gene Symbol

OLIG2

UniProt

Additional Olig2 Products

Product Documents for Human/Mouse Olig2 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse Olig2 Biotinylated Antibody

For research use only

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