Human/Mouse Olig3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB24561
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 257914
Product Specifications
Immunogen
E. coli-derived recombinant human Olig3
Met1-Lys272
Accession # Q7RTU3
Met1-Lys272
Accession # Q7RTU3
Specificity
Detects human Olig3 in direct ELISAs. Detects mouse Olig3 in Immunohistochemistry. In direct ELISA, no cross-reactivity with recombinant human (rh) Olig1 or rhOlig2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse Olig3 Antibody
Olig3 in 13 dpc Mouse Embryo Tissue.
Olig3 was detected in immersion fixed frozen sections of 13 dpc mouse embryo tissue using Mouse Anti-Human/Mouse Olig3 Monoclonal Antibody (Catalog # MAB24561) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse Olig3 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed frozen sections of 13 dpc mouse embryo tissue
Sample: Immersion fixed frozen sections of 13 dpc mouse embryo tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Olig3
Long Name
Oligodendrocyte Lineage Transcription Factor 3
Alternate Names
BHLHB7
Gene Symbol
OLIG3
UniProt
Additional Olig3 Products
Product Documents for Human/Mouse Olig3 Antibody
Product Specific Notices for Human/Mouse Olig3 Antibody
For research use only
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