Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody
Bio-Techne includes R&D Systems | Catalog # IC5246A
Discontinued Product
IC5246A has been discontinued and is replaced by
IC5246R.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 456738
Product Summary for Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody
Immunogen
NS0 mouse myeloma cell line transfected with human Orexin R2/HCRTR2
Met1-Trp444
Accession # AAC39602
Met1-Trp444
Accession # AAC39602
Specificity
Detects human and mouse Orexin R2/HCRTR2. Stains human and mouse Orexin R2/HCRTR2 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody
Detection of Orexin R2/HCRTR2 in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Mouse Anti-Human/Mouse Orexin R2/HCRTR2 APC-conjugated Monoclonal Antibody (Catalog # IC5246A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Orexin R2/HCRTR2 in Neuro‑2A Mouse Cell Line by Flow Cytometry.
Neuro-2A mouse neuroblastoma cell line was stained with Mouse Anti-Human/Mouse Orexin R2/HCRTR2 APC-conjugated Monoclonal Antibody (Catalog # IC5246A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: A172 human glioblastoma cell line and Neuro‑2A mouse neuroblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: A172 human glioblastoma cell line and Neuro‑2A mouse neuroblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Orexin R2/HCRTR2
Hypocretin Receptor 2 (HCRTR2), also known as Orexin Receptor 2 or OX2R, is a 40 kDa 7-transmembrane G-protein-coupled glycoprotein that is a high affinity receptor for Orexins A and B (Hypocretins 1 and 2). In mouse brain, engagement of HCRTRs promotes wakefulness, such that absence of either orexins or their receptors creates a narcolepsy-like state. It also influences reward circuits involving food or addictive drugs. The extracellular portions of human HCRTR2 share 92% and 93% aa identity with corresponding portions of mouse and rat HCRTR2, respectively.
Long Name
Hypocretin Receptor Type 2
Alternate Names
HCRTR2, Orexin Receptor 2, OrexinR2, OX2R
Gene Symbol
HCRTR2
UniProt
Additional Orexin R2/HCRTR2 Products
Product Documents for Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody
Product Specific Notices for Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody
For research use only
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