Detects human and mouse Pax3 and Pax7 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Pax1 is observed, approximately 10% with rhPax6, and less than 2% with rhPax2, rhPax4, rhPax5, and rhPax9.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human/Mouse Pax3 /Pax7 APC-conjugated Antibody

Detection of Pax3 in B16-F1 Mouse Cell Line by Flow Cytometry.

B16-F1 mouse melanoma cell line was stained with Mouse Anti-Human/Mouse Pax3 APC-conjugated Monoclonal Antibody (Catalog # IC2457A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse Pax3 /Pax7 APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: B16‑F1 mouse melanoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pax3/Pax7

Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. Human and mouse Pax3 share 98% amino acid sequence identity.

Long Name

Paired Box Gene 3 /Paired Box Gene 7

UniProt

NP_000429

Additional Pax3/Pax7 Products

Product Documents for Human/Mouse Pax3 /Pax7 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse Pax3 /Pax7 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human/Mouse Pax3 /Pax7 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC2457A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Pax3/Pax7 Antibodies »

Product Specifications

Immunogen

Specificity