Key Product Details

Species Reactivity

Human, Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 274212

View all Pax3/Pax7 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pax3 (isoform Pax3a)
Met1-Ser215
Accession # NP_000429

Specificity

Detects human and mouse Pax3 and Pax7 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Pax1 is observed, approximately 10% with rhPax6, and less than 2% with rhPax2, rhPax4, rhPax5, and rhPax9.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse Pax3 /Pax7 PE-conjugated Antibody

Detection of Pax3 antibody in B16-F1 Mouse Cell Line antibody by Flow Cytometry.

Detection of Pax3 in B16‑F1 Mouse Cell Line by Flow Cytometry.

B16-F1 mouse melanoma cell line was stained with Mouse Anti-Human/Mouse Pax3 PE-conjugated Monoclonal Antibody (Catalog # IC2457P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse Pax3 /Pax7 PE-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: B16‑F1 mouse melanoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Pax3/Pax7

Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. Human and mouse Pax3 share 98% amino acid sequence identity.

Long Name

Paired Box Gene 3 /Paired Box Gene 7

UniProt

NP_000429

Additional Pax3/Pax7 Products

Product Documents for Human/Mouse Pax3 /Pax7 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Pax3 /Pax7 PE-conjugated Antibody

For research use only

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