Human/Mouse Pax5/BSAP Alexa Fluor® 700-conjugated Antibody

Name: Human/Mouse Pax5/BSAP Alexa Fluor® 700-conjugated Antibody
Brand: R&D Systems
SKU: IC3487N

R&D Systems, part of Bio-Techne | Catalog # IC3487N

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Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

Key Product Details

Species Reactivity:	Human, Mouse
Immunogen:	E. coli-derived recombinant human Pax5 Thr141-His391 Accession # Q02548
Applications:	Flow Cytometry
Label:	Alexa Fluor 700 (Excitation=675-700 nm, Emission=723 nm)
Specificity:	Detects human Pax5 in direct ELISAs and Western blots.

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Technical Data
Citations / Reviews

Applications for Human/Mouse Pax5/BSAP Alexa Fluor® 700-conjugated Antibody

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	0.25-1 µg/10⁶ cells	Human peripheral blood mononuclear cells (PBMCs) fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Reconstitution Calculator

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Background: Pax5/BSAP

Pax5, also known as BSAP (B-cell-specific transcription factor) is a 42 kDa protein belonging to the paired box transcription factor family. It is a developmental regulator that is important for B-cell lineage commitment and development. Human Pax5 is a 391 amino acid (aa) protein containing the paired DNA-binding domain at the N‑terminal region. Several alternatively spliced isoforms with altered C‑terminal regions and possessing different transactivation properties have been described. All isoforms share the N‑terminal 235 aa with full-length Pax5. Human Pax5 shares 99%, 97% and 94% aa sequence identity with mouse, bovine and canine Pax5, respectively.

Item	Value
Long Name	Paired Box Gene 5/B-cell Specific Activator Protein
Entrez Gene IDs	5079 (Human); 18507 (Mouse)
Alternate Names	B cell specific activator protein, B-cell-specific transcription factor, BSAP, BSAPB-cell lineage specific activator, Pax5, paired box 5, paired box gene 5 (B-cell lineage specific activator protein), paired box gene 5 (B-cell lineage specific activator), paired box homeotic gene 5, paired box protein Pax-5, transcription factor PAX 5

Product Specific Notices for Human/Mouse Pax5/BSAP Alexa Fluor® 700-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human/Mouse Pax5/BSAP Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC3487N

Key Product Details

Applications for Human/Mouse Pax5/BSAP Alexa Fluor® 700-conjugated Antibody

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: Pax5/BSAP

Product Specific Notices for Human/Mouse Pax5/BSAP Alexa Fluor® 700-conjugated Antibody

Customer Reviews for Human/Mouse Pax5/BSAP Alexa Fluor® 700-conjugated Antibody

Product Documents for Human/Mouse Pax5/BSAP Alexa Fluor® 700-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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