Human/Mouse PIWIL1/HIWI Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6548
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PIWIL1/HIWI
Arg82-Thr290
Accession # Q96J94
Arg82-Thr290
Accession # Q96J94
Specificity
Detects human PIWIL1/HIWI in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse PIWIL1/HIWI Antibody
PIWIL1/HIWI in Mouse Testes.
PIWIL1/HIWI was detected in perfusion fixed frozen sections of mouse testes using Goat Anti-Human PIWIL1/HIWI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6548) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of spermatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse PIWIL1/HIWI Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse testes
Sample: Perfusion fixed frozen sections of mouse testes
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PIWIL1/HIWI
PIWI (P-element-induced wimpy testis; also HIWI and PIWIL1) is a 92-98 kDa member of the Piwi subfamily, Argonaute family of proteins. It is expressed in germline cells, particularly spermatocytes and spermatids, and also appears in CD34+ hematopoietic stem cells plus select tumor types. PIWI binds to a series of 28‑34 nucleic acid long noncoding RNAs that participate in gametogenesis. It does so under the influence of Arg methyltransferases, and in conjunction with Tudor‑containing proteins. Human PIWI/PIWIL1 is 861 amino acids (aa) in length. It contains a methylation target region in the N-terminus, followed by an RNA-binding PAZ domain (aa 277-391) and an RNA-cleaving PIWI domain (aa 555-847). There are two isoform variants. One is widely expressed and shows a three aa substitution for aa 1‑89. The second contains a six aa substitution for aa 824-861. Over aa 82-290, human PIWI (HIWI) shares 95% aa sequence identity with mouse PIWI (MIWI).
Long Name
Piwi-like Protein 1
Alternate Names
HIWI, MIWI
Entrez Gene IDs
9271 (Human)
Gene Symbol
PIWIL1
UniProt
Additional PIWIL1/HIWI Products
Product Documents for Human/Mouse PIWIL1/HIWI Antibody
Product Specific Notices for Human/Mouse PIWIL1/HIWI Antibody
For research use only
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