Human/Mouse PKA RII alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8000
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2394D
Product Specifications
Immunogen
Synthetic peptide containing Human/Mouse PKA RII alpha
Accession # P13861
Accession # P13861
Specificity
Detects human PKA RII alpha in direct ELISAs. Detects human and mouse PKA RII alpha in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse PKA RII alpha Antibody
Detection of Human PKA RII alpha by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PKA RIIa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PKA RII alpha in MDA-MB-231 Human Cell Line.
PKA RIIa was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
PKA RII alpha in Human Pancreatic Cancer Tissue.
PKA RIIa was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse PKA RII alpha Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line
Immunohistochemistry
0.3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
Western Blot
0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and PC‑3 human prostate cancer cell line
Sample: K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and PC‑3 human prostate cancer cell line
Reviewed Applications
Read 1 review rated 5 using MAB8000 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKA RII alpha
Long Name
cAMP-dependent Protein Kinase Regulatory Type II alpha
Alternate Names
PRKAR2A
Gene Symbol
PRKAR2A
UniProt
Additional PKA RII alpha Products
Product Documents for Human/Mouse PKA RII alpha Antibody
Product Specific Notices for Human/Mouse PKA RII alpha Antibody
For research use only
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