Human/Mouse R-Spondin 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4658
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 422407
Product Specifications
Immunogen
E. coli-derived recombinant human R-Spondin 1
Met1-Gly182
Accession # NP_775911
Met1-Gly182
Accession # NP_775911
Specificity
Detects human and mouse R-Spondin 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) R-Spondin 2 or rhR-Spondin 3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human/Mouse R-Spondin 1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human R-Spondin 1 (Catalog # 4645-RS)
Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS)
Sample: Recombinant Human R-Spondin 1 (Catalog # 4645-RS)
Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: R-Spondin 1
RSPO-1 (roof plate-specific spondin; also R-spondin1 and cristin3) is a 39 kDa, heparin-binding member of the Cristin/R-spondin family of proteins. These molecules apparently are either retained intracellularly or secreted, where they activate the frizzled-LRP receptor pathway. Mature human RSPO-1 is 27 kDa and 243 amino acids (aa) in length. It contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. A presumably glycosylated, 30 kDa, 193 aa C-terminally truncated form is secreted and interacts with heparin. The secreted form of human RSPO-1 is 93% aa identical to secreted mouse RSPO-1.
Long Name
Roof Plate-specific Spondin 1
Alternate Names
Cristin 3, CRISTIN3, HRspo1, RSPO, RSPO1, RSpondin 1
Gene Symbol
RSPO1
UniProt
Additional R-Spondin 1 Products
Product Documents for Human/Mouse R-Spondin 1 Antibody
Product Specific Notices for Human/Mouse R-Spondin 1 Antibody
For research use only
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