Human/Mouse/Rat 14-3-3 epsilon Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4419
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human 14-3-3 epsilon
Met1-Gln255
Accession # P62258
Met1-Gln255
Accession # P62258
Specificity
Detects human, mouse, and rat 14-3-3 epsilon in Western blots. In Western blots, less than 1% cross-reactivity with other recombinant human 14-3-3 isoforms is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat 14-3-3 epsilon Antibody
Detection of Human/Mouse/ Rat 14‑3‑3 epsilon by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. Specific bands for 14-3-3 epsilon was detected at approximately 26 and 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat 14-3-3 epsilon Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Sample: Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using AF4419 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 14-3-3 epsilon
14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha/ beta, epsilon, eta, gamma, tau/ theta, sigma, and zeta/ delta.
Alternate Names
1433 epsilon, YWHAE
Gene Symbol
YWHAE
UniProt
Additional 14-3-3 epsilon Products
Product Documents for Human/Mouse/Rat 14-3-3 epsilon Antibody
Product Specific Notices for Human/Mouse/Rat 14-3-3 epsilon Antibody
For research use only
Loading...
Loading...
Loading...
Loading...