Human/Mouse/Rat Akt2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2315
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 302514
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Akt2
Asn2-Glu481
Accession # P31751
Asn2-Glu481
Accession # P31751
Specificity
Detects human, mouse, and rat Akt2. Detects human Akt2 in direct ELISAs and endogenous Akt2 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Akt1 or rhAkt3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for Human/Mouse/Rat Akt2 Antibody
Detection of Human/Mouse/Rat Akt2 by Western Blot.
Western blot shows lysates of MDA-MB-468 human breast cancer cell line and Huh-7 human hepatoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse/Rat Akt2 Monoclonal Antibody (Catalog # MAB2315) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active Akt1 (Catalog # 1775-KS), recombinant human Akt2, and recombinant human Akt3 (2 ng/lane) were included. A specific band for Akt2 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse/Rat Akt2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: MDA-MB-468 human breast cancer cell line and Huh-7 human hepatoma cell line
Sample: MDA-MB-468 human breast cancer cell line and Huh-7 human hepatoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Akt2
The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKB alpha), Akt2 (PKB beta), and Akt3 (PKB gamma). Akt2 is expressed predominantly in insulin target tissues such as liver, skeletal muscle, and fat.
Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog 2
Alternate Names
PKB beta, RAC-beta
Gene Symbol
AKT2
UniProt
Additional Akt2 Products
Product Documents for Human/Mouse/Rat Akt2 Antibody
Product Specific Notices for Human/Mouse/Rat Akt2 Antibody
For research use only
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