Human/Mouse/Rat Akt2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF23151
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry-Paraffin, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Akt2
Accession # P31751
Accession # P31751
Specificity
Detects human, mouse, and rat Akt2 in Western blots. Also detects recombinant human Akt2 but not recombinant Akt1 or Akt3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Akt2 Antibody
Detection of Human/Mouse/Rat Akt2 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat Akt2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF23151) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active Akt1 (Catalog # 1775-KS), recombinant human Akt2, and recombinant human Akt3 (5 ng/lane) were included. A specific band for Akt2 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Akt2 in Rat Testis.
Akt2 was detected in perfusion fixed frozen sections of rat testis using Goat Anti-Human/Mouse/Rat Akt2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF23151) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Akt2 in Human Kidney Cancer Tissue.
Akt2 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 15 µg/mL Goat Anti-Human/Mouse/Rat Akt2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF23151) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat Akt2 Antibody
Application
Recommended Usage
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney cancer tissue, and perfusion fixed frozen sections of rat testis and mouse spleen
Sample: Immersion fixed paraffin-embedded sections of human kidney cancer tissue, and perfusion fixed frozen sections of rat testis and mouse spleen
Simple Western
5 µg/mL
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Western Blot
0.5 µg/mL
Sample: MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and U937 human histiocytic lymphoma cell line
Sample: MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and U937 human histiocytic lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF23151 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Akt2
The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKB alpha), Akt2 (PKB beta), and Akt3 (PKB gamma). Akt2 is expressed predominantly in insulin target tissues such as liver, skeletal muscle, and fat. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.
Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog 2
Alternate Names
PKB beta, RAC-beta
Gene Symbol
AKT2
UniProt
Additional Akt2 Products
Product Documents for Human/Mouse/Rat Akt2 Antibody
Product Specific Notices for Human/Mouse/Rat Akt2 Antibody
For research use only
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