Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4849
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human AlphaB Crystallin/CRYAB
Met1-Lys175
Accession # P02511
Met1-Lys175
Accession # P02511
Specificity
Detects human, mouse and rat AlphaB Crystallin/CRYAB in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CRYAA is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody
Detection of Human/Mouse/Rat AlphaB Crystallin/CRYAB by Western Blot.
Western blot shows lysates of mouse eye lens and human, mouse, and rat heart tissue. PVDF membrane was probed with 0.2 µg/mL Human/Mouse/Rat AlphaB Crystallin/CRYAB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4849) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human CRYAA and CRYAB (5 ng/lane) were included. A specific band for AlphaB Crystallin/CRYAB was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human and Mouse AlphaB Crystallin/CRYAB by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 29 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4849) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody
Application
Recommended Usage
Simple Western
2 µg/mL
Sample: Human heart tissue and mouse heart tissue
Sample: Human heart tissue and mouse heart tissue
Western Blot
0.2 µg/mL
Sample: Mouse eye lens and human, mouse, and rat heart tissue
Sample: Mouse eye lens and human, mouse, and rat heart tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AlphaB Crystallin/CRYAB
N‑terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved. There is also phosphorylation at Ser45 and 59, potential O-GlcNAc modification at Thr158, 162 or 170, and acetylation at Lys92. There is an alternate start site at Met68 that may be accompanied by a 47 aa substitution for aa 109‑175. Full-length human CRYAB is 54% aa identical to CRY-AlphaA, and 98% aa identical to mouse CRYAB.
Alternate Names
CRYA2, CRYAB, CTPP2, HSPB5
Gene Symbol
CRYAB
UniProt
Additional AlphaB Crystallin/CRYAB Products
Product Documents for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody
Product Specific Notices for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody
For research use only
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