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Human/Mouse/Rat Androgen R/NR3C4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB58761

R&D Systems, part of Bio-Techne
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MAB58761
MAB58761-SP

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 636005

Product Specifications

Immunogen

E. coli-derived recombinant human Androgen R/NR3C4
Thr660-Gln919
Accession # P10275

Specificity

Detects human Androgen R/NR3C4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat Androgen R/NR3C4 Antibody

Androgen R/NR3C4 antibody in Human Prostate by Immunohistochemistry (IHC-P).

Androgen R/NR3C4 in Human Prostate.

Androgen R/NR3C4 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human/Mouse/Rat Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58761) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse/Rat Androgen R/NR3C4 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Reviewed Applications

Read 1 review rated 5 using MAB58761 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Androgen R/NR3C4

The Androgen Receptor (AR) is a 90 kDa steroid hormone receptor that is critical for the development and function of the male reproductive system. AR binding to testosterone or 5 alpha-dihydrotestosterone (DHT) triggers receptor dimerization followed by translocation to the nucleus where it promotes transcription of androgen responsive genes. Multiple polymorphisms in AR are linked to the development of prostate cancer. The ligand binding domain of human AR (aa 661-920) shares 100% aa sequence identity with mouse and rat AR.

Long Name

Androgen Receptor

Alternate Names

AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1

Entrez Gene IDs

367 (Human)

Gene Symbol

AR

UniProt

Additional Androgen R/NR3C4 Products

Product Documents for Human/Mouse/Rat Androgen R/NR3C4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat Androgen R/NR3C4 Antibody

For research use only

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