Human/Mouse/Rat Annexin V Antibody
R&D Systems, part of Bio-Techne | Catalog # AF399
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Porcine
Applications
Validated:
Simple Western, Western Blot
Cited:
Flow Cytometry, Microarray, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Annexin V
Ala2-Asp320
Accession # P08758
Ala2-Asp320
Accession # P08758
Specificity
Detects human, mouse and rat Annexin V in Western blots. Detects human Annexin V in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Annexin V Antibody
Detection of Human, Mouse, and Rat Annexin V by Western Blot.
Western blot shows lysates of human lung tissue, mouse lung tissue, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Annexin V Antigen Affinity-purified Polyclonal Antibody (Catalog # AF399) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Annexin V at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human, Mouse, Rat Annexin V by Simple WesternTM.
Simple Western lane view shows lysates of Human lung, mouse lung, rat lung, loaded at 0.2 mg/mL. A specific band was detected for Annexin V at approximately 38 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human/Mouse/Rat Annexin V Antigen Affinity-purified Polyclonal Antibody (Catalog # AF399) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat Annexin V Antibody
Application
Recommended Usage
Simple Western
12.5 µg/mL
Sample: Human lung, mouse lung, rat lung
Sample: Human lung, mouse lung, rat lung
Western Blot
1 µg/mL
Sample: Human lung tissue, mouse lung tissue, and rat lung tissue
Sample: Human lung tissue, mouse lung tissue, and rat lung tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Annexin V
Additional Annexin V Products
Product Documents for Human/Mouse/Rat Annexin V Antibody
Product Specific Notices for Human/Mouse/Rat Annexin V Antibody
For research use only
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