Human/Mouse/Rat Calcineurin B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1348
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 212306
Product Specifications
Immunogen
E. coli-derived recombinant human Calcineurin B
Met1-Val170
Accession # P63098
Met1-Val170
Accession # P63098
Specificity
Detects endogenous human, mouse, and rat Calcineurin B.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat Calcineurin B Antibody
Detection of Human/Mouse/Rat Calcineurin B by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, TS1 mouse helper T cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Calcineurin B Monoclonal Antibody (Catalog # MAB1348) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Calcineurin B at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse/Rat Calcineurin B Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: MCF-7 human breast cancer cell line, TS1 mouse helper T cell line, and PC-12 rat adrenal pheochromocytoma cell line
Sample: MCF-7 human breast cancer cell line, TS1 mouse helper T cell line, and PC-12 rat adrenal pheochromocytoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB1348 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Calcineurin B
Background
Calcineurin, also called Protein Phosphatase 2B,
PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is
an enzyme that dephosphorylates serine and
threonine residues in proteins. It is a heterodimer of
a 59,000 dalton catalytic A subunit and a
1,900 dalton regulatory B subunit that is activated
by the binding of calcium ions and calmodulin (1).
Calcineurin is expressed in many tissues, but its
levels are highest in the brain, where it may play a
role in learning and memory (2). It has many
substrates, including NFAT, a transcription factor
that is activated by dephosphorylation (3).
Complexes of the immunosuppressants cyclosporin
and FK506 with immunophilin proteins such as
cyclophilin and FKBP12 are potent and specific
inhibitors of Calcineurin activity (4). Alterations in
Calcineurin activity are suspected to play a role in
cardiac hypertrophy (5) and graft versus host
disease in organ transplantation (6).
References:
1. Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.
2. Zeng H. et al. (2001) Cell 107:617.
3. Okamura, H. et al. (2000) Molecular Cell 6:539.
4. Liu, J. et al. (1992) Biochemistry 31:3896.
5. Molkentin, J.D. (2000) Circulation Research 87:731.
6. Sanquer, S.A. et al. (2004) Transplantation 77:854.
Alternate Names
CALNB1, Cnb1, MCIP1, PP2B beta 1, PPP3R1
Gene Symbol
PPP3R1
UniProt
Additional Calcineurin B Products
Product Documents for Human/Mouse/Rat Calcineurin B Antibody
Product Specific Notices for Human/Mouse/Rat Calcineurin B Antibody
For research use only
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