Human/Mouse/Rat Casein Kinase 1 gamma Antibody

Name: Human/Mouse/Rat Casein Kinase 1 gamma Antibody
Brand: R&D Systems
SKU: AF3186

R&D Systems, part of Bio-Techne | Catalog # AF3186

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Casein Kinase 1 gamma Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CK1 gamma
Val182-Tyr304
Accession # Q9HCP0

Specificity

Detects endogenous human, mouse, and rat CK1 gamma in Western blots. In Western blots, the immunogen is derived from a region of human CK1 gamma that is 97% to 98% identical to human CK1 gamma2 and CK1 gamma3, so all three isoforms should be detected.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Casein Kinase 1 gamma Antibody

Detection of Human/Mouse/Rat Casein Kinase 1 gamma by Western Blot.

Western blot shows lysates of human, mouse and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Casein Kinase 1 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3186) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CK1 gamma isoforms at approximately 45 to 51 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Casein Kinase 1 gamma in Human Brain.

Casein Kinase 1 gamma was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Sheep Anti-Human/Mouse/Rat Casein Kinase 1 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3186) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse/Rat Casein Kinase 1 gamma Antibody

Application

Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra)

Western Blot

1 µg/mL
Sample: Human, mouse and rat brain tissue

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Casein Kinase 1 gamma

Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, beta, gamma¹, gamma², gamma³, delta and epsilon) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. The C-termini of the gamma isoforms carry putative palmitoylation sites unique within the CK1 family that are believed to anchor the proteins in the plasma membrane.

Alternate Names

CSNK1G1

Entrez Gene IDs

53944 (Human); 214897 (Mouse); 64086 (Rat)

Gene Symbol