Human/Mouse/Rat CBP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2676
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rabbit
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Co-Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 257003
Product Specifications
Immunogen
E. coli-derived recombinant human CBP
Asn2141-Leu2442
Accession # Q92793
Asn2141-Leu2442
Accession # Q92793
Specificity
Detects human, mouse and rat CBP.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat CBP Antibody
Detection of Human, Mouse, and Rat CBP by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat CBP Monoclonal Antibody (Catalog # MAB2676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CBP at approximately 265 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CBP in HeLa Human Cell Line.
CBP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat CBP Monoclonal Antibody (Catalog # MAB2676) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse/Rat CBP Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line
Sample: HeLa human cervical epithelial carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CBP
The CBP (CREB-binding protein) is a histone acetyltransferase and transcriptional coactivator that regulates RNA polymerase II transcription. In addition to histone acetylation, CBP also acetylates various transcription factors, including the tumor suppressor protein p53, to enhance sequence-specific DNA binding.
Long Name
CREB-binding Protein
Alternate Names
CREBBP, RTS
Gene Symbol
CREBBP
UniProt
Additional CBP Products
Product Documents for Human/Mouse/Rat CBP Antibody
Product Specific Notices for Human/Mouse/Rat CBP Antibody
For research use only
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